WNT-5a, mouse

$ 245.00

Select Size:

  • W5A-M-005, 5 µg245.00
  • W5A-M-025, 25 µg1145.00
Add to Cart
Source M.W.~45 kDa CAS No.
Structural Info
FormulationLyophilized in sterile filtered solution of PBS with 2% CHAPS
ReconstitutionBefore reconstitution, we recommend a brief spin to drive down any material dislodged from the bottom of the tube.  The lyophilized protein should be reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of this protein, further dilutions should be made in buffer or medium containing carrier proteins, such as albumin or serum.
StabilityThe lyophilized protein is stable for at least 1 year if stored at -80 °C.  Reconstituted protein is stable for at least 1 month at 4 °C, but should be stored in aliquots at -80 °C for longer term. Avoid repeated freeze and thaw.
PurityGreater than 85% as determined by SDS-PAGE and HPLC analysis
Biological ActivityThe activity was determined by using a TCF reporter gene assay in 293 cells co-transfected with Frizzled-4 and LRP-5. WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay.
Country of OriginUSA

WNT-5a belongs to the class of WNT proteins that activate the “non-canonical” pathway.  The predicted size of mouse WNT-5a is a monomeric protein containing 356 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa by SDS-PAGE analysis under non-reducing conditions. StemRD’s product is expressed from a mouse cell line, and purified with a proprietary process that is distinct from the published method.

Ishida-Takagishi M, Enomoto A, et al., The Dishevelled-associating protein Daple controls the non-canonical Wnt/Rac pathway and cell motility. Nature Communication. 2012 May 29;3:859

Nakamuta S, Funahashi Y, Namba T et al., Local application of neurotrophins specifies axons through inositol 1,4,5-trisphosphate, calcium, and Ca2+/calmodulin-dependent protein kinases. Science Signal. 2011 Nov 15;4(199):ra76.