WNT-5a, human

$ 345.00

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  • W5A-H-005, 5 µg345.00
  • W5A-H-025, 25 µg1395.00
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Source M.W.~45 kDa CAS No.
Structural Info
FormulationLyophilized in sterile filtered solution of PBS with 1% CHAPS
ReconstitutionBefore reconstitution, we recommend a brief spin to drive down any material dislodged from the bottom of
the tube.  The lyophilized protein should be reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of this protein, further dilutions should be made in buffer or medium containing carrier proteins, such as albumin or serum.
StabilityThe lyophilized protein is stable for at least 6 months if stored at -80 °C.  Reconstituted protein is stable for at least 2
weeks at 4 °C, but should be stored in aliquots at -80 °C for longer term.  Avoid repeated freeze/thaw.
PurityGreater than 85% as determined by SDS-PAGE and HPLC analysis
Biological ActivityThe activity was determined by using a TCF reporter gene assay in 293 cells transfected with Frizzled-4 and LRP-5. WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay.
Country of OriginUSA

WNT-5a belongs to the class of WNT proteins that activate the “non-canonical” pathway. The predicted size of human WNT-5a is a monomeric protein containing 357 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa on SDS-PAGE under non-reducing conditions. StemRD’s product is expressed from a human cell line in animal-free medium, and purified with a proprietary process that is distinct from the published method.

Yamada A, Iwata T, Yamato M et al., Diverse functions of secreted frizzled-related proteins in the osteoblastogenesis of human multipotentmesenchymal stromal cells. Biomaterials. 2013 Apr;34(13):3270-8.