|Source||M.W.||~45 kDa||CAS No.|
|Formulation||Lyophilized in sterile filtered solution of PBS with 1% CHAPS|
|Reconstitution||Before reconstitution, we recommend a brief spin to drive down any material dislodged from the bottom of
the tube. The lyophilized protein should be reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of this protein, further dilutions should be made in buffer or medium containing carrier proteins, such as albumin or serum.
|Stability||The lyophilized protein is stable for at least 6 months if stored at -80 °C. Reconstituted protein is stable for at least 2
weeks at 4 °C, but should be stored in aliquots at -80 °C for longer term. Avoid repeated freeze/thaw.
|Purity||Greater than 85% as determined by SDS-PAGE and HPLC analysis|
|Biological Activity||The activity was determined by using a TCF reporter gene assay in 293 cells transfected with Frizzled-4 and LRP-5. WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay.|
|Country of Origin||USA|
WNT-5a belongs to the class of WNT proteins that activate the “non-canonical” pathway. The predicted size of human WNT-5a is a monomeric protein containing 357 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa on SDS-PAGE under non-reducing conditions. StemRD’s product is expressed from a human cell line in animal-free medium, and purified with a proprietary process that is distinct from the published method.