WNT-3a, human

$ 245.00

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  • W3A-H-005, 5 µg245.00
  • W3A-H-025, 25 µg1095.00
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Source M.W.38 - 41 kDa CAS No.
Structural Info
FormulationLyophilized in sterile filtered solution of PBS with 1% CHAPS
ReconstitutionBefore reconstitution, we recommend a brief spin to drive down any material dislodged from the bottom of the tube.  The lyophilized protein should be reconstituted in sterile H2O to a concentration of 100 ng/uL.  Because of the hydrophobic nature of this protein, further dilutions should be made in buffer or medium containing carrier proteins, such as albumin or serum.
StabilityThe lyophilized protein is stable for at least 6 months if stored at -80 °C.  Reconstituted protein is stable for at least
two weeks at 4 °C, but should be stored in aliquots at -80 °C for longer term.  Avoid repeated freeze and thaw.
PurityGreater than 90% as determined by SDS-PAGE and HPLC analysis
Biological ActivityThe activity was determined by using a TCF reporter gene assay in cultured human cells. The EC50 ranges from 50 - 150 ng/ml.
Country of OriginUSA

WNT-3a is a member of the WNT family of signaling proteins that play key roles in embryonic development and the maintenance of adult tissues. WNT-3a is a prototypic canonical WNT that signals through the b-catenin pathway.  The predicted size of human WNT-3a is a monomeric protein containing 328 amino acid residues.  Due to glycosylation, it migrates at an apparent molecular weight of 38 - 41 kDa on SDS-PAGE under non-reducing conditions.  StemRD’s WNT-3a is produced from a human cell line overexpressing human Wnt-3a cDNA in protein-free medium.  Purification is done by a proprietary process that is distinct from the published method.

Kim SE, Huang H, Zhao M, et al., Wnt Stabilization of β-Catenin Reveals Principles for Morphogen Receptor-Scaffold
Assemblies.  Science. 2013 May 17; 340(6134):867-70

Zhang X, Abreu JG, Yokota C, et al., Tiki1 is required for head formation via Wnt cleavage-oxidation and inactivation. Cell. 2012 Jun 22;149(7):1565-77.

Hoshiba T, Kawazoe N, Chen G. Mechanism of regulation of PPARG expression of mesenchymal stem cells by osteogenesis-mimickingextracellular matrices. Biosci Biotechnol Biochem. 2011;75(11):2099-104.