TGF-β1, human recombinant

$ 199.00

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  • TGFB1-005, 5 µg199.00
  • TGFB1-025, 25 µg749.00
  • TGFB1-100, 100 µg1999.00
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Source M.W.25 kDa CAS No.
Structural Info
FormulationLyophilized in sterile filtered solution of acetonitrile/TFA and PBS
ReconstitutionBefore reconstitution, we recommend a brief spin to drive down any material dislodged from the bottom of the tube.  The lyophilized protein should be reconstituted in 5 mM HCl. Further dilutions should be made in buffer or medium containing carrier proteins, such as albumin or serum.
StabilityThe lyophilized protein is stable for at least 1 year if stored at -80 °C.  Reconstituted protein is stable for at least 1
month at 4 °C, but should be stored in aliquots at -80 °C for longer term. Avoid repeated freeze and thaw.
PurityGreater than 95% as determined by SDS-PAGE and HPLC analyses
Biological ActivityThe activity was determined by using a SMAD reporter gene assay in human 293 cells. The EC50 ranges from 0.1 – 1.0 ng/ml.
Country of OriginUSA

One of the three closely-related members of the TGF-β family. Human TGF-β1 cDNA encodes a 390 aa precursor with a 29 aa signal peptide. A furin-like convertase cleaves the precursor to form a 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-β1. Disulfide-linked mature TGF-beta1 is dissociated from the LAP upon activation.
StemRD, produces active TGF-β1 in human 293 cells in Xeno-free medium. After a series of chromatography, the protein is purified to near homogeneity. Activity is determined by using a SMAD reporter gene assay with EC50 0.1 – 1.0 ng/mL.

HPLC and SDS-PAGE profiles of TGF-β1, human from StemRD Inc
tgfb, tgf b, tgf beta

Eyckmans J, Lin GL, Chen CS. Adhesive and mechanical regulation of mesenchymal stem Cell differentiation in human bone marrow andperiosteum-derived progenitor cells. Biol Open. 2012 Nov 15;1(11):1058-68.