Sonic Hedgehog, human

$ 235.00

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  • SHH-005, 5 µg235.00
  • SHH-025, 25 µg995.00
  • SHH-100, 100 µg3795.00
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SourceHuman Mammalian Cells M.W.~45kDa CAS No.
Structural Info
FormulationLyophilized in sterile filtered solution of PBS with 1% CHAPS
ReconstitutionBefore reconstitution, we recommend a brief spin to drive down any material dislodged from the bottom of
the tube.  The lyophilized protein should be reconstituted in sterile H2O to a concentration of 100 ng/uL.  Because of the hydrophobic nature of this protein, further dilutions should be made in buffer or medium containing carrier proteins, such as albumin or serum.
StabilityThe lyophilized protein is stable for at least 1 year if stored at -80 °C. Reconstituted protein is stable for at least 1 month at 4 °C, but should be stored in aliquots at -80 °C for longer term.  Avoid repeated freeze and thaw.
PurityGreater than 85% as determined by SDS-PAGE and HPLC analysis
Biological ActivityThe activity was determined by using a Gli reporter gene assay in 3T3 cells. The EC50 ranges from 5 to 20 ng/ml.
Country of OriginUSA

The hedgehog (Hh) gene encodes a secreted protein originally identified in Drosophila. The vertebrate homologs of Hh comprise of Sonic (SHh), Desert (DHh) and Indian (IHh).  SHh plays an important role in embryonic development and in diseases such as cancer.  Human SHh precursor is a 45kDa protein.  An autocatalytic reaction yields a 19 kDa N-terminal domain that is biologically active. Because of unique post-translational modifications, SHh produced in mammalian cells is much more biologically active than SHh produced from E. coli and is devoid of artifact associated with the E. coli products commercially available from other manufacturers.
SHh from StemRD made in human cells is 10-50 times more active, than other commercial products made in E. coli. Activity is detected by a Gli reporter gene assay in Swiss 3T3 cells.

Chen Y, Yue S, Xie L, et al., Dual Phosphorylation of suppressor of fused (Sufu) by PKA and GSK3beta regulates its stability and localizationin the primary cilium.  J Biol Chem. 2011 Apr 15;286(15):13502-11.

Ding H, Zhou D, Hao S, et al., Sonic hedgehog signaling mediates epithelial-mesenchymal communication and promotes renal fibrosis. J Am Soc Nephrol. 2012 May;23(5):801-13.

Wang J, Mook RA Jr, Lu J, et al., Identification of a novel Smoothened antagonist that potently suppresses Hedgehog signaling. Bioorg Med Chem. 2012 Nov 15;20(22):6751-7.

Okabe S, Tauchi T, Tanaka Y, et al., Effects of the hedgehog inhibitor GDC-0449, alone or in combination with dasatinib, on
BCR-ABL-positive leukemia cells. Stem Cells Dev. 2012 Nov 1;21(16):2939-48.