|Formulation||Lyophilized in sterile filtered solution of PBS.|
|Reconstitution||Before reconstitution, a brief spin is recommend to drive down any material dislodged from the bottom of the tube. The lyophilized protein should be reconstituted in sterile H2O to a desired concentration.
|Stability||The lyophilized protein is stable for at least one year if stored at -80 °C. Reconstituted protein is stable for at least four weeks at 4 °C, but should be stored in aliquots at -80 °C for longer term. Avoid repeated freeze and thaw.
|Purity||Greater than 90% as determined by SDS-PAGE analysis|
|Biological Activity||The activity was determined by using a TCF reporter gene assay in cultured human cells. The EC50 ranges from 2 - 10 ng/ml in the presence of 10 ng/mL human WNT-3a.|
|Country of Origin||USA|
R Spondin-2 is a natural enhancer of the canonical WNT pathway. When used together with WNT proteins that activate the beta-catenin pathway, Rspondin-1 enhances the activity of canonical WNT proteins by binding to LGR5 and LGR4 (refs.)
Refs: de Lau, et al, LGR5 homologues associate with Wnt receptors and mediate R-spondin signaling. Nature. 2011 July 4; 476: 293;
Carmon, et al, R-spondins function as ligands for the orphan receptors LGR4 and LGR5 to regulate Wnt/beta-catenin signaling. PNAS. 2011 Jul 12; 108: 11452.