|Source||E. coli||M.W.||25 kDa||CAS No.|
|Formulation||Lyophilized from sterile filtered PBS solution, carrier-free.|
|Reconstitution||Before reconstitution, we recommend a brief spin to drive down any material dislodged from the bottom of the tube. It is recommended to reconstitute the lyophilized product in acid solution such as 1mM HCl.
Further dilutions should be made in sterile buffer or medium containing carrier proteins, such as albumin or serum.
|Stability||The lyophilized protein is stable for at least 1 year if stored at -80 °C. Reconstituted protein is stable for at least 2 week at 4 ° C, but should be stored in aliquots at -80 °C for longer term. Avoid repeated freeze/thaw.|
|Purity||Greater than 95% by SDS-PAGE analysis|
|Biological Activity||The activity was determined by proliferation of the NIH3T3 cells. The EC50 ranges from 1 – 5 ng/ml.|
|Country of Origin||USA|
Platelet-Derived Growth Factor (PDGF) is a mitogen for many cell types, such as fibroblast, smooth muscle cells and endothelial cells. StemRD produces PDGF-bb in Escherichia coli as a disulfide bond-linked homodimer with a molecular mass of 25 kDa.
This product is suitable for tissue culture uses.